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Anti-HSD3a Antibody
Catalog #: 107-10392
HSD3a antibody [N1C2], 0.83 mg/ml (100 ul)
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 1109 |
| Protein Name / Synonyms | HSD3a |
| Concentration (lot specific) | 0.83 mg/ml |
| Recommended Applications | IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Synonyms
3-alpha-HSD antibody, C11 antibody, CDR antibody, CHDR antibody, DD4 antibody, HAKRA antibody, MGC22581 antibody, AKR1C4 antibody, dihydrodiol dehydrogenase isozyme DD4 antibody, type I 3-alpha-hydroxysteroid dehydrogenase antibody, 3-alpha-HSD1 antibody, aldo-keto reductase family 1 member C4 antibody, "aldo-keto reductase family 1, member C4 (chlordecone reductase, 3-alpha hydroxysteroid dehydrogenase, type I, dihydrodiol dehydrogenase 4) antibody"
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
37 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 217 of HSD3a (Uniprot ID# P17516)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
HSD3a antibody [N1C2] detects HSD3a protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HSD3a protein stained by HSD3a antibody [N1C2] (107-10392) diluted at 1:500.
Blue: Hoechst 33342 staining.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HSD3a protein stained by HSD3a antibody [N1C2] (107-10392) diluted at 1:500.
Blue: Hoechst 33342 staining.
Immunohistochemical analysis of paraffin-embedded gastric ca, using AKR1C4(107-10392) antibody at 1:100 dilution.
Sample (30 ug of whole cell lysate)
A: Hela
10% SDS PAGE
HSD3a antibody
107-10392 diluted at 1:1000
A: Hela
10% SDS PAGE
HSD3a antibody
107-10392 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.