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Anti-HSP10 Antibody
Catalog #: 130-10222
Mouse anti-HSP10(2E2-E7-D11) Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | P61604 |
| Gene Id | 3336 |
| Gene Symbols | HSPE1 |
| Clonality | Monoclonal |
| Immunogen | Immunogen was recombinant protein. This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with the immunogen. The IgG fraction of tissue culture supernatant was purified by Protein G/A affinity chromatography. |
| Recommended Applications | ELISA (recommended work dilution= 1:160, 000), Western Blotting (recommended work dilution= 1:1, 000-5, 000), |
| Antigen Catalog Number | 230-00231 |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Description
Heat shock 10 kDa protein 1 (Hsp10) also known as chaperonin 10 (cpn10) or early-pregnancy factor (EPF) is a protein that in humans is encoded by the HSPE1 gene. The homolog in E. coli is GroES that is a chaperonin which usually works in conjunction with GroEL.[1]GroES exists as a ring-shaped oligomer of between six to eight identical subunits, while the 60 kDa chaperonin (cpn60 - or groEL in bacteria) forms a structure comprising 2 stacked rings, each ring containing 7 identical subunits.[2] These ring structures assemble by self-stimulation in the presence of Mg2+-ATP. The central cavity of the cylindrical cpn60 tetradecamer provides an isolated environment for protein folding whilst cpn-10 binds to cpn-60 and synchronizes the release of the folded protein in an Mg2+-ATP dependent manner.[3] The binding of cpn10 to cpn60 inhibits the weak ATPase activity of cpn60.
Specificity
This antibody binds to its immunogen, and did not show any cross reactivity with unrelated antigens in ELISA. Cross reactivity with mouse and rat HSP10 has not been tested yet.
Storage
Store at 4°C if intended for use within one month, otherwise, store at -20°C to -80°C. The lyophilized antibody is stable for at least 18 months after the date of receipt when stored at -20°C to -80°C. After reconstitution, it can be aliquotted and stored at 4°C for 1 month or at -20°C to -80°C in a manual defrost freezer for many months without detectable loss activity.
Expiration
12 months from the date of shipment when stored properly.