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Anti-HSP22 (C-term) Antibody
Catalog #: 107-10487
HSP22 antibody [C2C3], C-Terminus, 1 mg/ml (100 ul)
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 26353 |
| Protein Name / Synonyms | HSP22, C-term |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Synonyms
CMT2L antibody, DHMN2 antibody, E2IG1 antibody, H11 antibody, HMN2 antibody, HMN2A antibody, HSP22 antibody, HSPB8 antibody, alpha-crystallin C chain antibody, protein kinase H11 antibody, small stress protein-like protein HSP22 antibody, heat shock 27kDa protein 8 antibody, heat shock protein beta-8 antibody, E2-induced gene 1 protein antibody, heat shock 22kDa protein 8 antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
22 kDa
Immunogen
Synthetic peptide corresponding to a region within amino acids 134 and 196 of HSP22 (Uniprot ID# Q9UJY1)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunohistochemical analysis of paraffin-embedded LUNG SCC, using HSP22(107-10487) antibody at 1:500 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using HSPB8(107-10487) antibody at 1:200 dilution.
Sample(30 ug whole cell lysate)
A:293T whole cell lysate
B:A431
12% SDS PAGE
107-10487 diluted at 1:1000
A:293T whole cell lysate
B:A431
12% SDS PAGE
107-10487 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.