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Anti-HSP27 Antibody
Catalog #: 130-10055
Rabbit Anti-Human HSP27 Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | P04792 |
| Gene Id | 3315 |
| Gene Symbols | HSPB1 HSP27 HSP28 |
| Clonality | Polyclonal |
| Immunogen | Immunogen was synthetic peptide derived from human HSP27. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum by ammonium sulphate precipitation, and followed by Protein A/G affinity chromatography. |
| Immunogen Sequence | CTERRVPFSLLRGPSWDDFRDWY |
| Recommended Applications | ELISA (recommended work dilution= 1: 20, 000(at least detecting 50 ng/ml)) |
| Reconstitution | Supplied as lyophilized and purified antibody originally containing PBS, without Preservative Stabilizers, such as Sodium Azide. The final concentration is indicated on the shipping vial. The antibody is stable for at least years from the data of receipt when stored at -20°C to -70°C. Reconstituted antibody (recommended with H20 1 mg/ml) can also be aliquoted and stored frozen at -20°C to -70°C in a manual defrost freezer for months without detectable loss activity. Upon reconstitution, the antibody can also be stored over months at 4°C. Please avoid freeze-thaw cycles. |
| Research Area | Angiogenesis, Cancer, Autophagy |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
HSP27 involved in stress resistance and actin organization.
General functions of HSP27 are the thermotolerance in vivo, the cytoprotection, and the support of cell survival under stress conditions. Special functions are manifold and complex. In vitro it acts as an ATPindependent chaperone by inhibiting protein aggregation and by stabilizing partially denatured proteins, which ensures refolding by the Hsp70-complex. HSP27 is also involved in the apoptotic signalling pathway which interacts with the outer mitochondrial membranes and interferes with the activation of cytochrome c/Apaf-1/dATP complex and therefore inhibits the activation of procaspase-9. A well documented function of HSP27 is the interaction with actin and intermediate filaments. It prevents the formation of non-covalent filament/filament interactions of the intermediate filaments and protects actin filaments from fragmentation. It also preserves the focal contacts fixed at the cell membrane.
Another function of HSP27 is the activation of the proteasome. It speeds up the degradation of irreversibly denatured proteins and junkproteins by binding to ubiquitinated proteins and to the 26S proteasome. HSP27 enhances the activation of the NF-?B pathway, that controls a lot of processes, such as cell growth and inflammatory and stress responses.
General functions of HSP27 are the thermotolerance in vivo, the cytoprotection, and the support of cell survival under stress conditions. Special functions are manifold and complex. In vitro it acts as an ATPindependent chaperone by inhibiting protein aggregation and by stabilizing partially denatured proteins, which ensures refolding by the Hsp70-complex. HSP27 is also involved in the apoptotic signalling pathway which interacts with the outer mitochondrial membranes and interferes with the activation of cytochrome c/Apaf-1/dATP complex and therefore inhibits the activation of procaspase-9. A well documented function of HSP27 is the interaction with actin and intermediate filaments. It prevents the formation of non-covalent filament/filament interactions of the intermediate filaments and protects actin filaments from fragmentation. It also preserves the focal contacts fixed at the cell membrane.
Another function of HSP27 is the activation of the proteasome. It speeds up the degradation of irreversibly denatured proteins and junkproteins by binding to ubiquitinated proteins and to the 26S proteasome. HSP27 enhances the activation of the NF-?B pathway, that controls a lot of processes, such as cell growth and inflammatory and stress responses.
Specificity
The antibody can specifically bind to its immunogen, and did not show any cross reactivity with unrelated antigens in ELISA. The specificity for binding to recombinant protein, cellular protein and native antigen is not defined. Cross reactivity with mouse and rat HSP70 has not yet been tested.
Storage
Keep it at 4°C if intended for use within a month. Store at -20°C if over months. Minimize freezing and thawing when use.
Related Products
Mouse Anti-Human Heat Shock Protein 27 (cat# DS-MB-01441) Recombinant Human Heat Shock Protein 27 (cat# 228-10789-3)
References
- Garrido C, et al..(2006) Heat shock proteins 27 and 70: anti-apoptotic proteins with tumorigenic properties. Cell Cycle. 5(22):2592-601.
- Didelot C, et al..(2006) Heat shock proteins: endogenous modulators of apoptotic cell death. Handb Exp Pharmacol. 172:171-98.
Expiration:
12 months from the date of shipment when stored properly.