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Anti-HSP60 Antibody
Catalog #: 130-10057
Rabbit Anti-Human HSP60 Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | P10809 |
| Gene Id | 3329 |
| Gene Symbols | HSPD1 HSP60 |
| Clonality | Polyclonal |
| Immunogen | Immunogen was synthetic peptide derived from human HSP60. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum by ammonium sulphate precipitation, and followed by Protein A/G affinity chromatography. |
| Immunogen Sequence | CAIATGGAVFGEEGLTLNLEDVQPHDLGK |
| Recommended Applications | ELISA (recommended work dilution= 1: 64, 000(at least detecting 16 ng/ml)) |
| Reconstitution | Supplied as lyophilized and purified antibody originally containing PBS, without Preservative Stabilizers, such as Sodium Azide. The final concentration is indicated on the shipping vial. The antibody is stable for at least years from the data of receipt when stored at -20°C to -70°C. Reconstituted antibody (recommended with H20 1 mg/ml) can also be aliquoted and stored frozen at -20°C to -70°C in a manual defrost freezer for months without detectable loss activity. Upon reconstitution, the antibody can also be stored over months at 4°C. Please avoid freeze-thaw cycles. |
| Research Area | Apoptosis, Cancer |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
HSP60 implicated in mitochondrial protein import and macromolecular assembly, facilitate the correct folding of imported proteins. HSP60 may also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. Defects in HSP60 are the cause of leukodystrophy hypomyelinating type 4 (HLD4); also called mitochondrial HSP60 chaperonopathy or MitCHAP-60 disease. HLD4 is a severe autosomal recessive hypomyelinating leukodystrophy. Clinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound mental retardation. As well as influencing apoptosis, HSP60 changes in expression level have been shown to be useful new biomarkers for diagnostic and prognostic purposes.
Specificity
The antibody can specifically bind to its immunogen, and did not show any cross reactivity with unrelated antigens in ELISA. The specificity for binding to recombinant protein, cellular protein and native antigen is not defined. Cross reactivity with mouse and rat HSP60 has not yet been tested.
Storage
Keep it at 4°C if intended for use within a month. Store at -20°C if not be used over months. Minimize freezing and thawing when use.
Related Products
Mouse Anti-Human Heat Shock Protein 60 (cat# DS-MB-01442 ) Custom Human HSP60 ELISA Kit available on demand (cat# ELH-HSP60-001 )
References
- Korányi L, et al..(2004) The role of hsp60 and hsp70 kDa heat shock protein families in different types of diabetes mellitus. Orv Hetil. 29;145(9):467-72.
- Tsan MF, Gao B. Cytokine function of heat shock proteins. Am J Physiol Cell Physiol. 2004 Apr;286(4):C739-44.
Expiration:
12 months from the date of shipment when stored properly.