Anti-HSPA5 (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Zebrafish
Host Species	Rabbit
Accession Number	P11021
Gene Id	3309
Gene Symbols	HSPA5
Protein Name / Synonyms	HSPA5 (Center)
Clonality	Polyclonal
Preparation	This HSPA5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 261-289 amino acids from the Central region of human HSPA5.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) [PubMed 8020977] pointed out that one of these, GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER). Because so many ER proteins interact transiently with GRP78, it may play a key role in monitoring protein transport through the cell.

Specificity

Predicted Reactivity

Mouse, Rat, Bovine, Chicken

Other Names

HSPA5; GRP78; 78 kDa glucose-regulated protein; Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; Heat shock 70 kDa protein 5; Immunoglobulin heavy chain-binding protein

NCBI Accession #

NP_005338.1

Other Accession #

P06761; P20029; Q90593; Q0VCX2

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

72333

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; IF: 1:10-50

	HSPA5 Antibody (Center) (Cat# 102-16232) western blot analysis in Hela,A2058,NCI-H460 cell line lysates (35ug/lane).This demonstrates the HSPA5 antibody detected the HSPA5 protein (arrow).
	HSPA5 Antibody (Center) (Cat# 102-16232) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HSPA5 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
	HSPA5 Antibody (Center) (Cat# 102-16232) western blot analysis in zebra fish brain tissue lysates (35ug/lane).This demonstrates the HSPA5 antibody detected the HSPA5 protein (arrow).
	Confocal immunofluorescent analysis of HSPA5 Antibody (Center)(Cat# 102-16232) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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