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Anti-Human-C-myc-Tag Antibody, Biotinylated
Catalog #: RB-10-0007B-50
Mouse anti--C-myc-Tag Biotinylated Antibody, 50 ug
Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Specificity | Myc-Tag antibody detects fusion recombinant proteins derived from a variety of sources at various concentrations. No cross reactivity was found with other epitope tags in ELISA or Western Blot. |
| Host Species | Mouse |
| Clonality | Monoclonal |
| Immunogen | Conjugated peptide with sequence EQKLISEEDL was used as immunogen. This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with the immunogen. The IgG fraction of tissue culture supernatant was purified by Protein G affinity chromatography. |
| Isotype | IgG1 |
| Recommended Applications | ELISA (recommended work dilution= 1:1000-5000), Western Blotting (recommended work dilution= 1-2 µg/ml) |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Plasmid vectors are commonly used for the expression of hybrid fusion proteins, encoding a protein of interest fused to an epitope tag. Antibodies specific for such fusion protein tags are useful for monitoring expression of the protein of interest, both in cells and in Western blot analysis. Such epitope tags in common use include Myc, GST, His, HA and GFP.
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
Myc-Tag antibody detects fusion recombinant proteins derived from a variety of sources at various concentrations. No cross reactivity was found with other epitope tags in ELISA or Western Blot.
Expiration:
12 months from the date of shipment when stored properly.