Human CD10 Antibody [clone: RFAL3], monoclonal mouse IgM. Validated for flow cytometry.
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Product Description
Specifications
| Size | 25 tests, 100 tests |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Mouse |
| Accession Number | P08473 |
| Gene Id | 4311 |
| Gene Symbols | MME, EPN |
| Protein Name / Synonyms | Neprilysin (EC 3.4.24.11) (Atriopeptidase) (Common acute lymphocytic leukemia antigen) (CALLA) (Enkephalinase) (Neutral endopeptidase 24.11) (NEP) (Neutral endopeptidase) (Skin fibroblast elastase) (SFE) (CD antigen CD10) |
| Clonality | Monoclonal |
| Clone | RFAL3 |
| Conjugation | RayBright® Blue 488 |
| Preparation | The antibody was purified by affinity chromatography. |
| Isotype | IgM |
| Format | Liquid |
| Concentration (lot specific) | 5 μl/test |
| Buffers | PBS, pH 7.2-7.4, 0.2% BSA, 0.1% sodium azide |
| Recommended Applications | Flow Cytometry |
| Research Area | Cardiovascular Disease |
| Excitation Laser | Blue Laser, 488 nm |
| Maximum Emission Wavelength | 518 nm, replace FITC, Alexa Fluor™ 488, and BD BB515 (Alexa Fluor™ is the trademark of Invitrogen) |
| Shipping Type | Blue ice |
| Storage | 4°C |
Product Features
- Serum-free media used for cell culture of hybridomas to minimize bovine antibody contamination
- Gentle elution at pH 4.0 to minimize antibody degradation
- Protein A, G & L affinity purification
- High purity
Typical Data
Human PBMCs were stained with RayBright® Blue 488 Anti-Human CD10 antibody (#136-14119, solid line), or mouse IgG isotype control (dashed line).
Description
CD10 is a type II transmembrane protein and is a member of the metalloprotease family. A neutral endopeptidase, CD10 is expressed on B cell precursors, T cell precursors, and neutrophils, and is involved in B cell development.
Recommended Usage
Each lot of antibody has been tested by flow cytometry staining. Researchers are recommended to further titrate this antibody at the optimal concentration for each specific application.
Storage / Stability
Store at 2 °C ~ 8 °C, protected from prolonged light exposure. DO NOT FREEZE.
