Human CD11 C Antibody [clone: 3.9], monoclonal mouse IgG1. Validated for flow cytometry.
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Product Description
Specifications
| Size | 100 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Mouse |
| Accession Number | P20702 |
| Gene Id | 3687 |
| Gene Symbols | ITGAX, CD11C |
| Protein Name / Synonyms | Integrin alpha-X (CD11 antigen-like family member C) (Leu M5) (Leukocyte adhesion glycoprotein p150,95 alpha chain) (Leukocyte adhesion receptor p150,95) (CD antigen CD11c) |
| Clonality | Monoclonal |
| Clone | 3.9 |
| Preparation | The antibody was purified by affinity chromatography. |
| Isotype | IgG1 |
| Format | Lyophilized |
| Concentration (lot specific) | Lyophilized |
| Buffers | PBS, pH 7.2-7.4; Carrier-free |
| Recommended Applications | Flow Cytometry |
| Reconstitution | Reconstitute in desired amount of PBS. |
| Research Area | Angiogenesis, Cardiovascular Disease |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Product Features
- Serum-free media used for cell culture of hybridomas to minimize bovine antibody contamination
- Gentle elution at pH 4.0 to minimize antibody degradation
- Protein A, G & L affinity purification
- High purity
- Carrier free
- Endotoxin-free
Typical Data
Human PBMCs were stained with either Purified Mouse Anti-Human CD11c primary antibody (#136-00137, blue histogram), or mouse IgG isotype control (gray histogram), followed by anti-mouse IgG/IgM RayBright® Blue 488 secondary antibody. Monocytes were gated.
Description
CD11c is a transmembrane glycoprotein, also referred to as integrin alpha X or complement receptor type 4 (CR4). CD11c reacts with an epitope on the α (150-kDa) chain of the CD11c/CD18 antigen complex. CD11c antigen is presented on monocytes, granulocytes and NK cells in peripheral blood.
Recommended Usage
Each lot of antibody has been pre-titrated and tested by flow cytometry staining. We suggest use 5 µl per 106 cells in 100 µl volume of staining buffer.
Storage / Stability
Store at 4°C if intended for use within one month. Otherwise, store at -20°C to -80°C.
