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Anti-Human CD151 Antibody, Purified
Catalog #: 136-00076
Human CD151 Antibody [clone: 50-6 [PTA-227]], monoclonal Mouse IgG1. Validated for flow cytometry.
Product Description
Specifications
| Size | 100 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Mouse |
| Protein Name / Synonyms | PETA-3, GP27, Membrane glycoprotein SFA-1, Tetraspanin-24, Tspan-24 |
| Clonality | Monoclonal |
| Clone | 50-6 [PTA-227] |
| Preparation | The antibody was purified by affinity chromatography. |
| Isotype | IgG1 |
| Format | Lyophilized |
| Concentration (lot specific) | Lyophilized |
| Buffers | PBS, pH 7.2-7.4; Carrier-free |
| Recommended Applications | Flow Cytometry |
| Reconstitution | Reconstitute in desired amount of PBS. |
| Research Area | Cardiovascular Disease |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Product Features
- Serum-free media used for cell culture of hybridomas to minimize bovine antibody contamination
- Gentle elution at pH 4.0 to minimize antibody degradation
- Protein A, G & L affinity purification
- High purity
- Carrier free
- Endotoxin-free
Typical Data
Human platelet-rich plasma were stained with either Purified Mouse Anti-Human CD151 primary antibody (#136-00076, blue histogram), or mouse IgG isotype control (gray histogram), followed by anti-mouse IgG/IgM RayBright R 647 secondary antibody.
Description
CD151 is a member of the tetraspanin family and is expressed by endothelium, epithelia, megakaryocytes, and platelets. Like others in this family, CD151 is made up of two extracellular loops, four transmembrane domains, and two cytoplasmic tails. CD151 has an effect on cell adhesion, migration, and tumor cell metastasis.
Recommended Usage
Each lot of antibody has been tested by flow cytometry staining. We suggest use ≤ 0.5 µg per million cells in 100 µl volume of staining buffer. Researchers are recommended to further titrate this antibody at the optimal concentration for each specific application.
Storage / Stability
Keep the lyophilized antibodies at -20 °C. Once reconstituted with PBS, store at 2 °C - 8 °C for less than 3 weeks or freeze at -20°C for long term storage. Please limit freeze and thaw cycles.