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Anti-Human CD154 (CD40L) Antibody, RayBright® Violet 450
Catalog #: 136-08036
Human CD154 (CD40L) Antibody [clone: 5c8], monoclonal mouse IgG2a kappa. Validated for flow cytometry.
Product Description
Specifications
| Size | 25 tests, 100 tests |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Mouse |
| Accession Number | P29965 |
| Gene Id | 959 |
| Gene Symbols | CD40LG, CD40L, TNFSF5, TRAP |
| Protein Name / Synonyms | CD40 ligand (CD40-L) (T-cell antigen Gp39) (TNF-related activation protein) (TRAP) (Tumor necrosis factor ligand superfamily member 5) (CD antigen CD154) [Cleaved into: CD40 ligand, membrane form; CD40 ligand, soluble form (sCD40L)] |
| Clonality | Monoclonal |
| Clone | 5c8 |
| Conjugation | RayBright® Violet 450 |
| Preparation | The antibody was purified by affinity chromatography. |
| Isotype | IgG2a Kappa |
| Format | Liquid |
| Concentration (lot specific) | 5 μl/test |
| Buffers | PBS, pH 7.2-7.4, 0.2% BSA, 0.1% sodium azide |
| Recommended Applications | Flow Cytometry |
| Research Area | Cardiovascular Disease, Inflammation |
| Excitation Laser | Violet Laser, 407 nm |
| Maximum Emission Wavelength | 454 nm, replace Pacific Blue™ |
| Shipping Type | Blue ice |
| Storage | 4°C |
Description
Product Features
- Serum-free media used for cell culture of hybridomas to minimize bovine antibody contamination
- Gentle elution at pH 4.0 to minimize antibody degradation
- Protein A, G & L affinity purification
- High purity
Description
CD154 is ligand of CD40, also named as CD40L, is a 32-39 kD type II transmembrane glycoprotein and a member of the TNF superfamily mainly expressed on activated T cells. CD40L binds CD40 on antigen presenting cells and enhances their activation, maturation and role for co-stimulation. CD40L is particularly important on a subset of T cells called T follicular helper cells (TFH cells). CD40L on TFH cell binds CD40 on B cell to induce its differentiation, isotype class-switching and protects B cells from apoptosis.
Recommended Usage
Each lot of antibody has been tested by flow cytometry staining. Researchers are recommended to further titrate this antibody at the optimal concentration for each specific application.
Storage / Stability
Store at 2 °C ~ 8 °C, protected from prolonged light exposure. DO NOT FREEZE.