Human CD2 Antibody [clone: 35.1], monoclonal mouse IgG2a. Validated for flow cytometry.
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Product Description
Specifications
| Size | 25 tests, 100 tests |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Mouse |
| Protein Name / Synonyms | CD2, LFA-2, T11, SRBC-R |
| Clonality | Monoclonal |
| Clone | 35.1 |
| Conjugation | RayBright® Blue 488 |
| Preparation | The antibody was purified by affinity chromatography. |
| Isotype | IgG2a |
| Format | Liquid |
| Concentration (lot specific) | 5 μl/test |
| Buffers | PBS, pH 7.2-7.4, 0.2% BSA, 0.1% sodium azide |
| Recommended Applications | Flow Cytometry |
| Research Area | Cardiovascular Disease |
| Excitation Laser | Blue Laser, 488 nm |
| Maximum Emission Wavelength | 518 nm, replace FITC, Alexa Fluor™ 488, and BD BB515 (Alexa Fluor™ is the trademark of Invitrogen) |
| Shipping Type | Blue ice |
| Storage | 4°C |
Product Features
- Serum-free media used for cell culture of hybridomas to minimize bovine antibody contamination
- Gentle elution at pH 4.0 to minimize antibody degradation
- Protein A, G & L affinity purification
- High purity
Typical Data
Human PBMCs were stained with RayBright® Blue 488 Anti-Human CD2 antibody (#136-14064, solid line), or mouse IgG isotype control (dashed line).
Description
CD2 is a transmembrane glycoprotein which is expressed on thymocytes, T lymphocytes, NK cells, and thymic B cell subsets. The main ligand for CD2 is CD58 and has a critical role in T cell activation, T cell signaling, and cell-cell adhesion.
Recommended Usage
Each lot of antibody has been tested by flow cytometry staining. Researchers are recommended to further titrate this antibody at the optimal concentration for each specific application.
Storage / Stability
Store at 2 °C ~ 8 °C, protected from prolonged light exposure. DO NOT FREEZE.
