Human CD279 (PD1) Antibody [clone: 5D5], monoclonal Mouse. Validated for flow cytometry.
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Product Description
Specifications
| Size | 100 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Mouse |
| Clonality | Monoclonal |
| Clone | 5D5 |
| Preparation | The antibody was purified by affinity chromatography. |
| Format | Lyophilized |
| Concentration (lot specific) | Lyophilized |
| Buffers | PBS, pH 7.2-7.4; Carrier-free |
| Recommended Applications | Flow Cytometry |
| Reconstitution | Reconstitute in desired amount of PBS. |
| Research Area | Cardiovascular Disease |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Product Features
- Serum-free media used for cell culture of hybridomas to minimize bovine antibody contamination
- Gentle elution at pH 4.0 to minimize antibody degradation
- Protein A, G & L affinity purification
- High purity
- Carrier free
- Endotoxin-free
Typical Data
PHA stimulated (3 days) human PBMCs were stained with either Purified Mouse Anti-Human PD-1 primary antibody (#136-00089, blue histogram), or mouse IgG isotype control (gray histogram), followed by anti-mouse IgG/IgM RayBright R 647 secondary antibody.
Description
CD279 is a 55 kD member of the immunoglobulin superfamily and contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic region. CD279 plays a key role in peripheral tolerance and autoimmune disease and is expressed in activated T cells, B cells, and myeloid cells. The ligands of CD279 include PD-L1 and PD-L2, and there is evidence that these ligands have overlapping functions and expression in normal tissues.
