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Anti-Human CD38 Antibody, Purified
Catalog #: 136-00046
Human CD38 Antibody [clone: THB-7[HB-7,HB7]], monoclonal Mouse IgG1 kappa. Validated for flow cytometry.
Product Description
Specifications
| Size | 100 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Mouse |
| Protein Name / Synonyms | CD38, ADP-ribosyl cyclase |
| Clonality | Monoclonal |
| Clone | THB-7[HB-7, HB7] |
| Preparation | The antibody was purified by affinity chromatography. |
| Isotype | IgG1 Kappa |
| Format | Lyophilized |
| Concentration (lot specific) | Lyophilized |
| Buffers | PBS, pH 7.2-7.4; Carrier-free |
| Recommended Applications | Flow Cytometry |
| Reconstitution | Reconstitute in desired amount of PBS. |
| Research Area | Cardiovascular Disease |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Product Features
- Serum-free media used for cell culture of hybridomas to minimize bovine antibody contamination
- Gentle elution at pH 4.0 to minimize antibody degradation
- Protein A, G & L affinity purification
- High purity
- Carrier free
- Endotoxin-free
Typical Data
Human PBMCs were stained with either Purified Mouse Anti-Human CD38 primary antibody (#136-00046, blue histogram), or mouse IgG isotype control (gray histogram), followed by AF 647 anti-mouse IgG/IgM secondary antibody.
Description
CD38, a 45 kD type II transmembrane glycoprotein, is an ADP-ribosyl hydrolase expressed at variable levels on hematopoietic cells. In humans, it is highly expressed on hematopoietic cells including early B and T cell lineages, activated B and T cells, NK cells, monocytes, and dendritic cells. CD38 functions as signaling channel which leads to cellular activation and proliferation. CD38 also plays a role as an ectoenzyme with various functions as well as an inducer of Ca2+ mobilization from cytoplasmic stores, and modulate immune cell activation and proliferation.
Recommended Usage
Each lot of antibody has been tested by flow cytometry staining. We suggest use ≤ 0.5 µg per million cells in 100 µl volume of staining buffer. Researchers are recommended to further titrate this antibody at the optimal concentration for each specific application.
Storage / Stability
Keep the lyophilized antibodies at -20 °C. Once reconstituted with PBS, store at 2 °C - 8 °C for less than 3 weeks or freeze at -20°C for long term storage. Please limit freeze and thaw cycles.