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Anti-Human CD45RO Antibody, Purified
Catalog #: 136-00139
Human CD45RO Antibody [clone: UCHL-1], monoclonal mouse IgG2a. Validated for flow cytometry.
Product Description
Specifications
| Size | 100 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Mouse |
| Protein Name / Synonyms | CD45RO, CD45R, PTPRC, LCA, GP180 |
| Clonality | Monoclonal |
| Clone | UCHL-1 |
| Preparation | The antibody was purified by affinity chromatography. |
| Isotype | IgG2a |
| Format | Lyophilized |
| Concentration (lot specific) | Lyophilized |
| Buffers | PBS, pH 7.2-7.4; Carrier-free |
| Recommended Applications | Flow Cytometry |
| Reconstitution | Reconstitute in desired amount of PBS. |
| Research Area | Cardiovascular Disease |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Product Features
- Serum-free media used for cell culture of hybridomas to minimize bovine antibody contamination
- Gentle elution at pH 4.0 to minimize antibody degradation
- Protein A, G & L affinity purification
- High purity
- Carrier free
- Endotoxin-free
Description
CD45RO is a splice variant of tyrosine phosphatase CD45, a type I transmembrane glycoprotein. It is used to subdivide T cell populations based on their expression. The CD45RO isoform is expressed on activated and memory T cells, some B cell subsets, activated monocytes/macrophages, and granulocytes. It has also been reported to bind galectin-1 and CD22.
Recommended Usage
Each lot of antibody has been pre-titrated and tested by flow cytometry staining. We suggest use 5 µl per 106 cells in 100 µl volume of staining buffer.
Storage / Stability
Store at 4°C if intended for use within one month. Otherwise, store at -20°C to -80°C.