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Anti-Human CD57 Antibody, Purified
Catalog #: 136-00070
Human CD57 Antibody [clone: HNK-1 [HNK1, Leu7]], monoclonal Mouse Igm. Validated for flow cytometry.
Product Description
Specifications
| Size | 100 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Mouse |
| Protein Name / Synonyms | HNK-1, NK-1, Leu-7 |
| Clonality | Monoclonal |
| Clone | HNK-1 [HNK1, Leu7] |
| Preparation | The antibody was purified by affinity chromatography. |
| Isotype | IgM |
| Format | Lyophilized |
| Concentration (lot specific) | Lyophilized |
| Buffers | PBS, pH 7.2-7.4; Carrier-free |
| Recommended Applications | Flow Cytometry |
| Reconstitution | Reconstitute in desired amount of PBS. |
| Research Area | Cardiovascular Disease |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Product Features
- Serum-free media used for cell culture of hybridomas to minimize bovine antibody contamination
- Gentle elution at pH 4.0 to minimize antibody degradation
- Protein A, G & L affinity purification
- High purity
- Carrier free
- Endotoxin-free
Description
CD57 is an oligosaccharide antigenic determinant which is expressed on various proteins, lipids, and chondroitin sulfate proteoglycans. While its function is largely unknown, it is thought that CD57 may have some involvement in cell-matrix interactions. CD57 tends to be increased in some diseases where CD4/CD8 are imbalanced.
Recommended Usage
Each lot of antibody has been tested by flow cytometry staining. We suggest use ≤ 0.5 µg per million cells in 100 µl volume of staining buffer. Researchers are recommended to further titrate this antibody at the optimal concentration for each specific application.
Storage / Stability
Keep the lyophilized antibodies at -20 °C. Once reconstituted with PBS, store at 2 °C - 8 °C for less than 3 weeks or freeze at -20°C for long term storage. Please limit freeze and thaw cycles.