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Anti-Human CD7 Antibody, Purified
Catalog #: 136-00138
Human CD7 Antibody [clone: T3-3A1], monoclonal mouse IgG1 kappa. Validated for flow cytometry.
Product Description
Specifications
| Size | 100 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Mouse |
| Protein Name / Synonyms | CD7, Leu-9, TP41, Tp40, GP40 |
| Clonality | Monoclonal |
| Clone | T3-3A1 |
| Preparation | The antibody was purified by affinity chromatography. |
| Isotype | IgG1 Kappa |
| Format | Lyophilized |
| Concentration (lot specific) | Lyophilized |
| Buffers | PBS, pH 7.2-7.4; Carrier-free |
| Recommended Applications | Flow Cytometry |
| Reconstitution | Reconstitute in desired amount of PBS. |
| Research Area | Cardiovascular Disease |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Product Features
- Serum-free media used for cell culture of hybridomas to minimize bovine antibody contamination
- Gentle elution at pH 4.0 to minimize antibody degradation
- Protein A, G & L affinity purification
- High purity
- Carrier free
- Endotoxin-free
Typical Data
Human PBMCs were stained with either Purified Mouse Anti-Human CD7 primary antibody (#136-00138, blue histogram), or mouse IgG isotype control (gray histogram), followed by anti-mouse IgG/IgM RayBright® Blue 488 secondary antibody.
Description
CD7 is a type I transmembrane glycoprotein, also referred to as gp40. It is expressed on thymocytes, T cells, pre-B cells, and NK cells. Co-stimulating of CD7 induces cytokine secretion and modulate cellular adhesion. Crosslinking of CD7 induces transmembrane calcium flux in T cells.
Recommended Usage
Each lot of antibody has been pre-titrated and tested by flow cytometry staining. We suggest use 5 µl per 106 cells in 100 µl volume of staining buffer.
Storage / Stability
Store at 4°C if intended for use within one month. Otherwise, store at -20°C to -80°C.