Human CD7 Antibody [clone: T3-3A1], monoclonal mouse IgG1 kappa. Validated for flow cytometry.
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Product Description
Specifications
| Size | 25 tests, 100 tests |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Mouse |
| Protein Name / Synonyms | CD7, Leu-9, TP41, Tp40, GP40 |
| Clonality | Monoclonal |
| Clone | T3-3A1 |
| Conjugation | RayBright® Blue 488 |
| Preparation | The antibody was purified by affinity chromatography. |
| Isotype | IgG1 Kappa |
| Format | Liquid |
| Concentration (lot specific) | 5 μl/test |
| Buffers | PBS, pH 7.2-7.4, 0.2% BSA, 0.1% sodium azide |
| Recommended Applications | Flow Cytometry |
| Research Area | Cardiovascular Disease |
| Excitation Laser | Blue Laser, 488 nm |
| Maximum Emission Wavelength | 518 nm, replace FITC, Alexa Fluor™ 488, and BD BB515 (Alexa Fluor™ is the trademark of Invitrogen) |
| Shipping Type | Blue ice |
| Storage | 4°C |
Product Features
- Serum-free media used for cell culture of hybridomas to minimize bovine antibody contamination
- Gentle elution at pH 4.0 to minimize antibody degradation
- Protein A, G & L affinity purification
- High purity
Typical Data
Human PBMCs were stained with RayBright® Blue 488 Anti-Human CD45RO antibody (#136-14138, solid line), or mouse IgG isotype control (dashed line). CD3 positive cells were gated.
Description
CD7 is a type I transmembrane glycoprotein, also referred to as gp40. It is expressed on thymocytes, T cells, pre-B cells, and NK cells. Co-stimulating of CD7 induces cytokine secretion and modulate cellular adhesion. Crosslinking of CD7 induces transmembrane calcium flux in T cells.
Recommended Usage
Each lot of antibody has been tested by flow cytometry staining. Researchers are recommended to further titrate this antibody at the optimal concentration for each specific application.
Storage / Stability
Store at 2 °C ~ 8 °C, protected from prolonged light exposure. DO NOT FREEZE.
