Product Description

Specifications

Size	25 tests, 100 tests
Estimated Lead Time	1-2 business days
Species	Human
Host Species	Mouse
Gene Symbols	CD8
Protein Name / Synonyms	CD8, cluster of differentiation 8, T8, Leu2
Clonality	Monoclonal
Clone	OKT8
Conjugation	APC
Preparation	The antibody was purified by affinity chromatography.
Isotype	IgG2a Kappa
Format	Liquid
Concentration (lot specific)	5 μl/test
Buffers	PBS, pH 7.2-7.4, 0.2% BSA, 0.1% sodium azide
Recommended Applications	Flow Cytometry
Research Area	Cardiovascular Disease, Wnt-beta-Catenin Signaling
Excitation Laser	Red Laser, 633 nm
Maximum Emission Wavelength	676 nm
Shipping Type	Blue ice
Storage	4°C

Product Features

Serum-free media used for cell culture of hybridomas to minimize bovine antibody contamination
Gentle elution at pH 4.0 to minimize antibody degradation
Protein A, G & L affinity purification
High purity

Typical Data

Human PBMCs were stained with APC Anti-Human CD8 antibody (#136-25025, solid line), or mouse IgG isotype control (dashed line).

Description

CD8 (cluster of differentiation 8) also known as T8 and Leu2, is a surface glycoprotein comprising of α and β chains, which are expressed on immature thymocytes, mature CD8 T cells and some other cell types, e.g., dendritic cells. CD8 exist as either an α/β heterodimer in most thymocytes or α/α homodimer on a subset of γ/δ TCR-bearing intraepithelial lymphocytes. CD8 is an antigen coreceptor on T cells that associates with the T cell receptor complex during antigen presentation by APC cells through MHC class I. This antibody recognizes the 34 kDa CD8 α chain on the cell surface.

Recommended Usage

Each lot of antibody has been tested by flow cytometry staining. Researchers are recommended to further titrate this antibody at the optimal concentration for each specific application.

Storage / Stability

Store at 2 °C ~ 8 °C, protected from prolonged light exposure. DO NOT FREEZE.