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Anti-Human CD8a Antibody, Biotinylated
Catalog #: 136-01025
Biotinylated Human CD8a Antibody [clone: OKT8], monoclonal Mouse IgG2a kappa. Validated for flow cytometry.
Product Description
Specifications
| Size | 100 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Mouse |
| Gene Symbols | CD8 |
| Protein Name / Synonyms | cluster of differentiation 8, T8, Leu2 |
| Clonality | Monoclonal |
| Clone | OKT8 |
| Conjugation | Biotin |
| Preparation | The antibody was purified by affinity chromatography, and conjugated with biotin |
| Isotype | IgG2a Kappa |
| Format | Liquid |
| Concentration (lot specific) | 0.5 mg/ml |
| Buffers | PBS, pH 7.2-7.4, 0.2% BSA, 0.1% sodium azide |
| Recommended Applications | Flow Cytometry |
| Research Area | Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | 4°C |
Description
Product Features
- Serum-free media used for cell culture of hybridomas to minimize bovine antibody contamination
- Gentle elution at pH 4.0 to minimize antibody degradation
- Protein A, G & L affinity purification
- High purity
Description
CD8 (cluster of differentiation 8) also known as T8 and Leu2, is a surface glycoprotein comprising of α and β chains, which are expressed on immature thymocytes, mature CD8 T cells and some other cell types, e.g., dendritic cells. CD8 exist as either an α/β heterodimer in most thymocytes or α/α homodimer on a subset of γ/δ TCR-bearing intraepithelial lymphocytes. CD8 is an antigen coreceptor on T cells that associates with the T cell receptor complex during antigen presentation by APC cells through MHC class I. This antibody recognizes the 34 kDa CD8 α chain on the cell surface.
Recommended Usage
Each lot of antibody has been tested by flow cytometry staining. We suggest use ≤ 0.5 µg per million cells in 100 µl volume of staining buffer. Researchers are recommended to further titrate this antibody at the optimal concentration for each specific application.
Storage / Stability
Stored at 2 °C - 8 °C, protected from prolonged light exposure. DO NOT FREEZE.