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Anti-Human CD8a Antibody, RayBright® Violet 500
Catalog #: 136-09025
Human CD8a Antibody [clone: OKT8], monoclonal mouse IgG2a kappa. Validated for flow cytometry.
Product Description
Specifications
| Size | 25 tests, 100 tests |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Mouse |
| Gene Symbols | CD8 |
| Protein Name / Synonyms | CD8, cluster of differentiation 8, T8, Leu2 |
| Clonality | Monoclonal |
| Clone | OKT8 |
| Conjugation | RayBright® Violet 500 |
| Preparation | The antibody was purified by affinity chromatography. |
| Isotype | IgG2a Kappa |
| Format | Liquid |
| Concentration (lot specific) | 5 μl/test |
| Buffers | PBS, pH 7.2-7.4, 0.2% BSA, 0.1% sodium azide |
| Recommended Applications | Flow Cytometry |
| Research Area | Cardiovascular Disease |
| Excitation Laser | Violet Laser, 407 nm |
| Maximum Emission Wavelength | 537 nm, replace Brilliant Violet 510™ (Brilliant Violet 510™ is a trademark of BD) |
| Shipping Type | Blue ice |
| Storage | 4°C |
Description
Product Features
- Serum-free media used for cell culture of hybridomas to minimize bovine antibody contamination
- Gentle elution at pH 4.0 to minimize antibody degradation
- Protein A, G & L affinity purification
- High purity
Description
CD8 (cluster of differentiation 8) also known as T8 and Leu2, is a surface glycoprotein comprising of α and β chains, which are expressed on immature thymocytes, mature CD8 T cells and some other cell types, e.g., dendritic cells. CD8 exist as either an α/β heterodimer in most thymocytes or α/α homodimer on a subset of γ/δ TCR-bearing intraepithelial lymphocytes. CD8 is an antigen coreceptor on T cells that associates with the T cell receptor complex during antigen presentation by APC cells through MHC class I. This antibody recognizes the 34 kDa CD8 α chain on the cell surface.
Recommended Usage
Each lot of antibody has been tested by flow cytometry staining. Researchers are recommended to further titrate this antibody at the optimal concentration for each specific application.
Storage / Stability
Store at 2 °C ~ 8 °C, protected from prolonged light exposure. DO NOT FREEZE.