Human IFN-gamma Antibody [clone: gamma3-11.1], monoclonal mouse IgG1. Validated for flow cytometry.
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Product Description
Specifications
| Size | 25 tests, 100 tests |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Mouse |
| Protein Name / Synonyms | IFNƴ, Interferon gamma |
| Clonality | Monoclonal |
| Clone | gamma3-11.1 |
| Conjugation | RayBright® Red 647 |
| Preparation | The antibody was purified by affinity chromatography. |
| Isotype | IgG1 |
| Format | Liquid |
| Concentration (lot specific) | 5 μl/test |
| Buffers | PBS, pH 7.2-7.4, 0.2% BSA, 0.1% sodium azide |
| Recommended Applications | Flow Cytometry |
| Research Area | Cardiovascular Disease |
| Excitation Laser | Red Laser, 633 nm |
| Maximum Emission Wavelength | 664 nm, replace Cy5™ and Alexa Fluor™ 647 (Cy5™ and Alexa Fluor™ are the trademark of Invitrogen and GE Health Care) |
| Shipping Type | Blue ice |
| Storage | 4°C |
Product Features
- Serum-free media used for cell culture of hybridomas to minimize bovine antibody contamination
- Gentle elution at pH 4.0 to minimize antibody degradation
- Protein A, G & L affinity purification
- High purity
Typical Data
PMA + ionomycin stimulated human PBMCs were stained with RayBright® Red 647 Anti-Human IFN-γ antibody (#136-24077, left), or mouse IgG isotype control (right).
Description
IFNƴ is multifunctional cytokine mostly produced by NK cells and T cells. IFNƴ has anti-proliferative, immunoregulatory, and proinflammatory effects as well as anti-viral activities. It induces the anti-microbial and anti-tumour responses of macrophages, NK cells and neutrophils.
Recommended Usage
Each lot of antibody has been tested by flow cytometry staining. Researchers are recommended to further titrate this antibody at the optimal concentration for each specific application.
Storage / Stability
Store at 2 °C ~ 8 °C, protected from prolonged light exposure. DO NOT FREEZE.
