3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-Human-JNK (p183/185) Antibody
Catalog #: RB-02-0016
Rabbit anti-Human-JNK (p183/185) Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Specificity | This antibody binds to its immunogen, and did not show any cross reactivity with unrelated antigens in ELISA. Cross reactivity with mouse and rat JNK(p183/185) has not yet been tested. |
| Accession Number | P45983 |
| Gene Id | 5599 |
| Gene Symbols | MAPK8 JNK1 PRKM8 SAPK1 SAPK1C |
| Clonality | Polyclonal |
| Immunogen | The imunogen was synthetic peptide. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum followed by Protein A/G affinity chromatography. |
| Immunogen Sequence | CSFMMTPY(P)VVTR |
| Recommended Applications | ELISA (recommended work dilution= 1:2, 000) |
| Reconstitution | Product is supplied as a powder obtained from lyophilization of purified antibody in PBS without preservatives. Reconstitute the antibody with sterile water to a final concentration of 1 mg/ml. |
| Research Area | MAPK Signaling, Autophagy |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
The insulin signaling pathway plays a pivotal role in lifespan regulation and stress resistance in many diverse organisms. Components of the JNK (c-Jun N-terminal kinase) signaling pathway have been demonstrated to interact with the insulin signaling pathway. The JNK family is a subgroup of the mitogen-activated protein kinase superfamily and is part of a signal transduction cascade that is activated by cytokines and by exposure to environmental stresses. The p38 MAPKs are also activated by lipopolysaccharide. Dysregulation of MAPK kinase pathways has been associated with various diseases including cancer (ERK), neurodegeneration (JNK) and inflammation (p38).
Typical Data
Storage/Stability
Store at 4°C if intended for use within one month, otherwise, store at -20°C to -80°C. The lyophilized antibody is stable for at least 18 months after the date of receipt when stored at -20°C to -80°C. After reconstitution, it can be aliquoted and stored frozen at -20°C to -80°C in a manual defrost freezer for at least 6 months without detectable loss of activity. Upon reconstitution, the antibody can also be stored for 1 month at 4°C. Please avoid multiple freeze-thaw cycles, as this will lower the activity of the antibody.
Expiration
12 months from the date of shipment when stored properly.
References
- Wang J, et al.. (2011) Defective anchoring of JNK1 in the cytoplasm by MKK7 in Jurkat cells is associated with resistance to Fas-mediated apoptosis. Mol Biol Cell. 22(1):117-27.
- Antonyak MA, et al.. (2002) Elevated JNK activation contributes to the pathogenesis of human brain tumors. Oncogene. 21(33):5038-46.