RayBright® Red 780 Human MHC class I Antibody [clone: W6/32], monoclonal Mouse IgG2a kappa. Validated for flow cytometry.
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Product Description
Specifications
| Size | 25 tests, 100 tests |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Mouse |
| Protein Name / Synonyms | MHC class I |
| Clonality | Monoclonal |
| Clone | W6/32 |
| Conjugation | RayBright® Red 780 |
| Preparation | The antibody was purified by affinity chromatography, and conjugated with a high quality fluorochrome, RayBright® Red 780 |
| Isotype | IgG2a Kappa |
| Format | Liquid |
| Concentration (lot specific) | 5 μl/test |
| Buffers | PBS, pH 7.2-7.4, 0.2% BSA, 0.1% sodium azide |
| Recommended Applications | Flow Cytometry |
| Research Area | Cardiovascular Disease |
| Excitation Laser | Red Laser, 633 nm |
| Maximum Emission Wavelength | 780 nm, replace APC-Alexa Fluor™ 750 tandems |
| Shipping Type | Blue ice |
| Storage | 4°C |
Product Features
- Serum-free media used for cell culture of hybridomas to minimize bovine antibody contamination
- Gentle elution at pH 4.0 to minimize antibody degradation
- Protein A, G & L affinity purification
- High purity
Typical Data
Description
MHC class I molecules are expressed on the cell surface of all nucleated cells in humans. This antibody recognizes human HLA-A, B, C. MHC class I consists of three α units (α1, α2, and α3) and one β unit (β2 microglobulin). These units interact with a CD8 T cell receptor complex and present non-self antigenic peptide which triggers an immediate response from the immune system against a particular non-self antigen. Human MHC class I play an important role in cell-mediated immune responses and tumor surveillance.
Product Details
Recommended Usage
Each lot of antibody has been pre-titrated and tested by flow cytometry staining. We suggest use 5 µl per 106 cells in 100 µl volume of staining buffer.
Storage/Stability
Stored at 2°C ~ 8°C, protected from prolonged light exposure. DO NOT FREEZE.
