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Anti-Human TNF-alpha Antibody, APC
Catalog #: 136-25056
Human TNF-alpha Antibody, monoclonal mouse IgG2b. Validated for flow cytometry.
Product Description
Specifications
| Size | 25 tests, 100 tests |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Mouse |
| Protein Name / Synonyms | TNF-alpha, Cachectin, Necrosin, Macrophage cytotoxic factor (MCF), Differentiation inducing factor (DIF), TNFSF2 |
| Clonality | Monoclonal |
| Conjugation | APC |
| Preparation | The antibody was purified by affinity chromatography. |
| Isotype | IgG2b |
| Format | Liquid |
| Concentration (lot specific) | 5 μl/test |
| Buffers | PBS, pH 7.2-7.4, 0.2% BSA, 0.1% sodium azide |
| Recommended Applications | Flow Cytometry |
| Research Area | Cardiovascular Disease, Inflammation, Wnt-beta-Catenin Signaling |
| Excitation Laser | Red Laser, 633 nm |
| Maximum Emission Wavelength | 676 nm |
| Shipping Type | Blue ice |
| Storage | 4°C |
Description
Product Features
- Serum-free media used for cell culture of hybridomas to minimize bovine antibody contamination
- Gentle elution at pH 4.0 to minimize antibody degradation
- Protein A, G & L affinity purification
- High purity
Description
Monomeric human TNF-alpha is a non-glycosylated 157 amino acid protein which is secreted by secreted by macrophages, monocytes, neutrophils, T-cells, and NK-cells. TNF-alpha often forms multimeric complexes with trimers being most common in solution. TNF-alpha can bind to surface receptors and cause an array of activities to take place including lysis of neighboring tumor cells and virus infected cells, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.
Recommended Usage
Each lot of antibody has been tested by flow cytometry staining. Researchers are recommended to further titrate this antibody at the optimal concentration for each specific application.
Storage / Stability
Store at 2 °C ~ 8 °C, protected from prolonged light exposure. DO NOT FREEZE.