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Anti-Human TNF-alpha Antibody, Biotinylated
Catalog #: 136-01056
Biotinylated Human TNF-alpha Antibody, monoclonal Mouse IgG2b. Validated for flow cytometry.
Product Description
Specifications
| Size | 100 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Mouse |
| Protein Name / Synonyms | Cachectin, Necrosin, Macrophage cytotoxic factor (MCF), Differentiation inducing factor (DIF), TNFSF2 |
| Clonality | Monoclonal |
| Conjugation | Biotin |
| Preparation | The antibody was purified by affinity chromatography. |
| Isotype | IgG2b |
| Format | Liquid |
| Concentration (lot specific) | 0.5 mg/ml |
| Buffers | PBS, pH 7.2-7.4, 0.2% BSA, 0.1% sodium azide |
| Recommended Applications | Flow Cytometry |
| Research Area | Cardiovascular Disease, Inflammation |
| Shipping Type | Blue ice |
| Storage | 4°C |
Description
Product Features
- Serum-free media used for cell culture of hybridomas to minimize bovine antibody contamination
- Gentle elution at pH 4.0 to minimize antibody degradation
- Protein A, G & L affinity purification
- High purity
Description
Monomeric human TNF-alpha is a non-glycosylated 157 amino acid protein which is secreted by secreted by macrophages, monocytes, neutrophils, T-cells, and NK-cells. TNF-alpha often forms multimeric complexes with trimers being most common in solution. TNF-alpha can bind to surface receptors and cause an array of activities to take place including lysis of neighboring tumor cells and virus infected cells, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.
Recommended Usage
Each lot of antibody has been tested by flow cytometry staining. We suggest use ≤ 0.5 µg per million cells in 100 µl volume of staining buffer. Researchers are recommended to further titrate this antibody at the optimal concentration for each specific application.
Storage / Stability
Stored at 2 °C - 8 °C, protected from prolonged light exposure. DO NOT FREEZE.