HUS1 antibody, 0.71 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 3364 |
| Protein Name / Synonyms | HUS1 |
| Concentration (lot specific) | 0.71 mg/ml |
| Recommended Applications | IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
hHUS1 antibody, HUS1 antibody, checkpoint protein HUS1 antibody, hus1+-like protein antibody, HUS1 checkpoint homolog (S. pombe) antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
32 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 273 of HUS1 (Uniprot ID# O60921)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
HUS1 antibody detects HUS1 protein at cytosol and nucleus on mouse lymph node by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lymph node.
HUS1 antibody (107-11089) dilution: 1:500.
Sample: Paraffin-embedded mouse lymph node.
HUS1 antibody (107-11089) dilution: 1:500.
HUS1 antibody detects HUS1 protein at cytosol and nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
HUS1 antibody (107-11089) dilution: 1:500.
Sample: Paraffin-embedded rat fore brain.
HUS1 antibody (107-11089) dilution: 1:500.
HUS1 antibody detects HUS1 protein by Western blot analysis.
A. 30 µg C8D30 whole cell lysate/extract
12 % SDS-PAGE
HUS1 antibody (107-11089) dilution: 1:1000
A. 30 µg C8D30 whole cell lysate/extract
12 % SDS-PAGE
HUS1 antibody (107-11089) dilution: 1:1000
Immunohistochemical analysis of paraffin-embedded CA922 xenograft , using HUS1(107-11089) antibody at 1:500 dilution.
Sample (30 ug of whole cell lysate)
A: 293T
12% SDS PAGE
107-11089 diluted at 1:1000
A: 293T
12% SDS PAGE
107-11089 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.