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Anti-Hyper-Phospho-Tau (pSer262) Antibody
Catalog #: RB-02-0023
Mouse anti-Hyper-phosphorylated-Tau (pSer262) Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Specificity | The antibody specifically detects phosphorylated protein derived from human brain tissue. It recognizes PHF-tau phosphorylated at Ser262 in both ELISA and WB. This antibody shows no cross-reactivity with other unrelated protein. |
| Accession Number | NP_058518 |
| Clonality | Monoclonal |
| Clone | 13G6.D3 |
| Immunogen | Immunogen was synthetic peptide derived from human Tau. This antibody was obtained from a mice immunized with the immunogen, then fusion, selection and identification. The identified clone was called as 13G6.D3. This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells deriveded from the immunized mouse. The IgG fraction of cell culture supernatant was purified by Protein G affinity chromatography. |
| Format | Lyophilized |
| Recommended Applications | ELISA (recommended work dilution= 1: 1, 000-2, 000), Western Blotting (recommended work dilution= 2-5 µg/ml), Immunoprecipitation |
| Reconstitution | Sterile PBS (pH 7.5) |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Microtubule-associated protein, tau is abnormally hyperphosphorylated in the brain of patients with Alzheimer's disease, and is the major protein subunit of paired helical filaments. There is also a significant pool of non-paired helical filament abnormally phosphorylated tau in Alzheimer's disease brain. Tau is a family of six isoforms, derived from a single gene by mRNA splicing. Tau protein is produced by a single gene expressed predominantly in neurons. They vary in size from 352-441 amino acides. In Alzheimer disease Tau is hyperphosphorylated, containing 3-4 fold more phosphoate/mole of the protein than the normal tau. Recent investigations show that MARK and PKA phosphorylate several sites within the repeats (notably the KXGS motifs including Ser262, Ser324, and Ser356, plus Ser320); in addition PKA phosphorylates some sites in the flanking domains, notably Ser214. This type of phosphorylation strongly reduces tau's affinity for microtubules, and at the same time inhibits tau's assembly into PHFs (Paired helical filament).
Typical Data
Storage/Stability
Always prepare single-use aliquots planning use while receiving package. A small amount of antibody can be kept at 4°C for daily use. For a long storage, recommend to keep them at -20°C after adding an equal volume of glycerol in PBS. It will not lose activity over a year. Minimize freezing and thawing when use.
Expiration
12 months from the date of shipment when stored properly.
References
- Arima, K., et al. (2000). NACP/alpha-synuclein and tau constitute two distinctive subsets of filaments in the same neuronal inclusions in brains from a family of parkinsonism and dementia with Lewy bodies: double-immunolabeling fluorescence and electron microscope studies. Acta Neuropathol. 100(2), 115-121.
- Gong CX, Grundke-Iqbal I, Iqbal K(1994).Dephosphorylation of Alzheimer's disease abnormally phosphorylated tau by protein phosphatase-2A.Neuroscience. 61(4):765-72.
- Biernat, J., et al. (1992). The switch of tau protein to an Alzheimer-like state includes the phosphorylation of two serine-proline motifs upstream of the microtubule binding region. EMBO J. 11(4), 1593-1597.