Synonyms
I-309 antibody, P500 antibody, SCYA1 antibody, SISe antibody, TCA3 antibody, CCL1 antibody, small inducible cytokine A1 (I-309, homologous to mouse Tca-3) antibody, C-C motif chemokine 1 antibody, inflammatory cytokine I-309 antibody, small-inducible cytokine A1 antibody, T lymphocyte-secreted protein I-309 antibody, chemokine (C-C motif) ligand 1 antibody
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
11 kDa
Immunogen
Synthetic peptide corresponding to a region within amino acids 32 and 96 of I-309 (Uniprot ID# P22362)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

I-309 antibody detects CCL1 protein at cytosol on Breast Carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded Breast Carcinoma.
I-309 antibody (107-10764) dilution: 1:250.

Sample(30 µg of whole cell lysate)
A:MOLT4
15% SDS PAGE
107-10764 diluted at 1:500
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.