Iba1 antibody, 0.06 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 199 |
| Protein Name / Synonyms | Iba1 |
| Concentration (lot specific) | 0.06 mg/ml |
| Recommended Applications | IHC (Formalin-fixed paraffin-embedded sections), Immunohistochemistry, Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
AIF-1 antibody, IBA1 antibody, IRT-1 antibody, IRT1 antibody, AIF1 antibody, ionized calcium-binding adapter molecule 1 antibody, allograft inflammatory factor 1 antibody, interferon gamma responsive transcript antibody, protein G1 antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
1XPBS, 1%BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
17 kDa
Immunogen
Synthetic peptide corresponding to a region within amino acids 85 and 147 of Iba1 (Uniprot ID# P55008)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Immunohistochemistry,Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunohistochemical analysis of paraffin-embedded Hepatoma, using Iba1(107-10175) antibody at 1:500 dilution.
Iba1 detects Iba1 protein at cytosol on mouse brain tissue by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain tissue.
Iba1 (107-10175) dilution: 1:1000.
Sample: Paraffin-embedded mouse brain tissue.
Iba1 (107-10175) dilution: 1:1000.
Iba1 antibody detects Iba1 protein by western blot analysis.
A. 30 µg THP-1 whole cell lysate/extract
B. 30 µg HL-60 whole cell lysate/extract
15 % SDS-PAGE
Iba1 antibody (107-10175) dilution: 1:1000
A. 30 µg THP-1 whole cell lysate/extract
B. 30 µg HL-60 whole cell lysate/extract
15 % SDS-PAGE
Iba1 antibody (107-10175) dilution: 1:1000
Iba1 antibody detects Iba1 protein by Western blot analysis.
A. 50 µg mouse brain lysate/extract
15 % SDS-PAGE
Iba1 antibody (107-10175) dilution: 1:1000
A. 50 µg mouse brain lysate/extract
15 % SDS-PAGE
Iba1 antibody (107-10175) dilution: 1:1000
Iba1 antibody detects Iba1 protein on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
Iba1 antibody (107-10175) dilution: 1:500.
Sample: Paraffin-embedded mouse fore brain.
Iba1 antibody (107-10175) dilution: 1:500.
Iba1 antibody detects Iba1 protein on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
Iba1 antibody (107-10175) dilution: 1:500.
Sample: Paraffin-embedded rat hind brain.
Iba1 antibody (107-10175) dilution: 1:500.
Iba1 antibody detects Iba1 protein at cytoplasm on colon carcinoma stroma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma stroma.
Iba1 antibody (107-10175) dilution: 1:500.
Sample: Paraffin-embedded colon carcinoma stroma.
Iba1 antibody (107-10175) dilution: 1:500.
Sample (30 ug of whole cell lysate)
A: Molt-4
15% SDS PAGE
AIF1 antibody
107-10175 diluted at 1:1000
A: Molt-4
15% SDS PAGE
AIF1 antibody
107-10175 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.