Anti-ID1 (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P41134
Gene Id	3397
Gene Symbols	ID1
Protein Name / Synonyms	ID1 (Center)
Clonality	Polyclonal
Preparation	This ID1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-93 amino acids from the Central region of human ID1.
Concentration (lot specific)	0.500 mg/ml
Recommended Applications	Western Blotting, FC
Research Area	Angiogenesis, Cardiovascular Disease
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

The protein encoded by this gene is a helix-loop-helix (HLH) protein that can form heterodimers with members of the basic HLH family of transcription factors. The encoded protein has no DNA binding activity and therefore can inhibit the DNA binding and transcriptional activation ability of basic HLH proteins with which it interacts. This protein may play a role in cell growth, senescence, and differentiation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

Specificity

Predicted Reactivity

Rat

Other Names

ID1; BHLHB24; ID; DNA-binding protein inhibitor ID-1; Class B basic helix-loop-helix protein 24; Inhibitor of DNA binding 1

NCBI Accession #

NP_002156.2;NP_851998.1

Other Accession #

P41135; P20067; NP_851998.1; NP_002156.2

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

16133

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50; IF: 1:10-50; FC: 1:10-50

	ID1 Antibody (Center) (Cat# 102-11226) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the ID1 antibody detected the ID1 protein (arrow).
	ID1 Antibody (Center)(Cat# 102-11226) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the ID1(PEI 1:100) antibody detected the ID1 protein (arrow).
	ID1 Antibody (Center) (Cat# 102-11226)immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ID1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
	Confocal immunofluorescent analysis of ID1 Antibody (Center)(Cat# 102-11226) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
	ID1 Antibody (Center) (Cat# 102-11226) flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.