Rabbit Anti-ID4 (N-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | P47928 |
| Gene Id | 3400 |
| Gene Symbols | ID4 |
| Protein Name / Synonyms | ID4 (N-term) |
| Clonality | Polyclonal |
| Preparation | This ID4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human ID4. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | FC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
ID4 is transcription factors containing a basic helix-loop-helix (bHLH) motif regulate expression of tissue-specific genes in a number of mammalian and insect systems. DNA-binding activity of the bHLH proteins is dependent on formation of homo- and/or heterodimers. Dominant-negative HLH proteins encoded by Id-related genes, such as ID4, also contain the HLH-dimerization domain but lack the DNA-binding basic domain. Consequently, Id proteins inhibit binding to DNA and transcriptional transactivation by heterodimerization with bHLH proteins
Specificity
Predicted Reactivity
Pig
Other Names
ID4; BHLHB27; DNA-binding protein inhibitor ID-4; Class B basic helix-loop-helix protein 27; Inhibitor of DNA binding 4
NCBI Accession #
NP_001537.1
Other Accession #
Q06AV5; P41139
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
16622
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100; FC: 1:10-50
 | Western blot analysis of ID4 Antibody (N-term) (Cat# 102-18468) in mouse bladder tissue lysates (35ug/lane). ID4 (arrow) was detected using the purified Pab. |
 | ID4 Antibody (N-term) (Cat# 102-18468) IHC analysis in formalin fixed and paraffin embedded bladder carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ID4 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated. |
 | ID4 Antibody (N-term) (Cat# 102-18468) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.
