Anti-IDH1 (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	O75874
Gene Id	3417
Gene Symbols	IDH1
Protein Name / Synonyms	IDH1 (Center)
Clonality	Polyclonal
Preparation	This IDH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 116-143 amino acids from the Central region of human IDH1.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	IF, IHC-P, Western Blotting, FC
Research Area	Cancer
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

IDH1 belongs to two distinct subclasses. The protein is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. This protein contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production.

Specificity

Predicted Reactivity

Rat, Bovine

Other Names

IDH1; PICD; Isocitrate dehydrogenase [NADP] cytoplasmic; Cytosolic NADP-isocitrate dehydrogenase; IDP; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase

NCBI Accession #

NP_001269315.1;NP_001269316.1;NP_005887.2

Other Accession #

P41562; O88844; Q9XSG3

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

46659

Recommended Dilutions

IF: 1:10-50; IHC: 1:50-100; WB: 1:1000; FC: 1:10-50

	Confocal immunofluorescent analysis of IDH1 Antibody (Center)(Cat# 102-17359) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
	Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with IDH1 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	Western blot analysis of IDH1 Antibody (Center) (Cat# 102-17359) in HepG2 cell line and mouse liver tissue lysates (35ug/lane). IDH1 (arrow) was detected using the purified Pab.
	IDH1 Antibody (Center) (Cat# 102-17359) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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