Anti-IDH3G (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P51553
Gene Id	3421
Gene Symbols	IDH3G
Protein Name / Synonyms	IDH3G (C-term)
Clonality	Polyclonal
Preparation	This IDH3G antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 366-393 amino acids from the C-terminal region of human IDH3G.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, FC
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the gamma subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. This gene is a candidate gene for periventricular heterotopia.

Specificity

Predicted Reactivity

Bovine, Monkey

Other Names

IDH3G; Isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial; Isocitric dehydrogenase subunit gamma; NAD(+)-specific ICDH subunit gamma

NCBI Accession #

NP_004126.1;NP_777358.1

Other Accession #

P41564; Q58CP0

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

42794

Recommended Dilutions

WB: 1:1000; FC: 1:10-50

	Western blot analysis of lysate from human placenta tissue lysate, using IDH3G Antibody (C-term)(Cat# 102-18397). 102-18397 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.
	IDH3G Antibody (C-term) (Cat# 102-18397) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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