Synonyms
CIG-49 antibody, GARG-49 antibody, IFI60 antibody, IFIT4 antibody, IRG2 antibody, ISG60 antibody, RIG-G antibody, IFIT3 antibody, IFIT-3 antibody, retinoic acid-induced gene G protein antibody, interferon-induced protein with tetratricopeptide repeats 4 antibody, ISG-60 antibody, CIG49 antibody, IFI-60K antibody, IFIT-4 antibody, interferon-induced protein with tetratricopeptide repeats 3 antibody, interferon-induced 60 kDa protein antibody
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
56 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 230 and 490 of IFIT3 (Uniprot ID# O14879)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000[suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunofluorescence analysis of methanol-fixed HeLa, using IFIT3(107-11025) antibody at 1:500 dilution.

Immunohistochemical analysis of paraffin-embedded Hela xenograft, using IFIT3(107-11025) antibody at 1:500 dilution.

Sample (30 ug of whole cell lysate)
A: 293T
7.5% SDS PAGE
107-11025 diluted at 1:5000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.