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Anti-IFN-gamma Antibody, Biotinylated
Catalog #: 130-10166B-50
Mouse anti-Interferon gamma (IFNG) Biotinylated Antibody, 50 ug
Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Mouse |
| Accession Number | P01579 |
| Gene Id | 3458 |
| Gene Symbols | IFNG |
| Clonality | Monoclonal |
| Immunogen | Immunogen was recombinant protein. This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with the immunogen. The IgG fraction of tissue culture supernatant was purified by Protein G/A affinity chromatography. |
| Isotype | IgG1 |
| Recommended Applications | ELISA (recommended work dilution= 1:640, 000), Western Blotting (recommended work dilution= 1:1, 000-5, 000), |
| Antigen Catalog Number | 230-00210 |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Research Area | Cancer, Inflammation, Autophagy, Innate Immunity |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Interferon gamma (IFN?) is a dimerized soluble cytokine that is the only member of the type II class of interferons.[2] The existence of this interferon, which early in its history was known as immune interferon, was recognized in 1970[3] when tuberculin-sensitized peritoneal cells were challenged with PPD and resulting supernatants were shown to inhibit growth of vesicular stomatitis virus. That report also contained the basic observation underlying the now widely employed interferon gamma release assay used to test for TB. This interferon was later called macrophage-activating factor, a term now used to describe a larger family of proteins to which IFN? belongs. In humans, the IFN? protein is encoded by the IFNG gene.
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
This antibody binds to its immunogen, and did not show any cross reactivity with unrelated antigens in ELISA. Cross reactivity with mouse and rat IFN? has not been tested yet.
Expiration:
12 months from the date of shipment when stored properly.