Human IFN-gamma Antibody [clone: gamma3-11.1], monoclonal mouse IgG1. Validated for flow cytometry.
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Product Description
Specifications
| Size | 25 tests, 100 tests |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Mouse |
| Protein Name / Synonyms | IFNƴ, Interferon gamma |
| Clonality | Monoclonal |
| Clone | gamma3-11.1 |
| Conjugation | RayBright® Blue 488 |
| Preparation | The antibody was purified by affinity chromatography. |
| Isotype | IgG1 |
| Format | Liquid |
| Concentration (lot specific) | 5 μl/test |
| Buffers | PBS, pH 7.2-7.4, 0.2% BSA, 0.1% sodium azide |
| Recommended Applications | Flow Cytometry |
| Research Area | Cardiovascular Disease |
| Excitation Laser | Blue Laser, 488 nm |
| Maximum Emission Wavelength | 518 nm, replace FITC, Alexa Fluor™ 488, and BD BB515 (Alexa Fluor™ is the trademark of Invitrogen) |
| Shipping Type | Blue ice |
| Storage | 4°C |
Product Features
- Serum-free media used for cell culture of hybridomas to minimize bovine antibody contamination
- Gentle elution at pH 4.0 to minimize antibody degradation
- Protein A, G & L affinity purification
- High purity
Typical Data
PMA + ionomycin stimulated human PBMCs were stained with RayBright® Blue 488 Anti-Human IFN-γ antibody (#136-14077, solid line), or mouse IgG isotype control (dashed line). CD3 positive cells were gated.
Description
IFNƴ is multifunctional cytokine mostly produced by NK cells and T cells. IFNƴ has anti-proliferative, immunoregulatory, and proinflammatory effects as well as anti-viral activities. It induces the anti-microbial and anti-tumour responses of macrophages, NK cells and neutrophils.
Recommended Usage
Each lot of antibody has been tested by flow cytometry staining. Researchers are recommended to further titrate this antibody at the optimal concentration for each specific application.
Storage / Stability
Store at 2 °C ~ 8 °C, protected from prolonged light exposure. DO NOT FREEZE.
