RayBiotech

3607 Parkway Ln Ste 200 Norcross, Georgia 30092

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ISO 13485:2016 cGMP

Anti-IFT81 Antibody

Catalog #: 144-61394

IFT81 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
Q8WYA0
Gene Id
28981
Gene Symbols
IFT81
Protein Name / Synonyms
IFT81, CDV-1, CDV-1R, CDV1, CDV1R, DV1, intraflagellar transport 81
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting
Research AreaCardiovascular Disease
Shipping TypeBlue ice
Storage-20°C

Description

Background

The protein encoded by this gene, together with IFT74, forms a tubulin-binding module of intraflagellar transport complex B. This module is involved in transport of tubulin within the cilium, and the encoded protein is required for ciliogenesis. Mutations in this gene are a cause of short-rib polydactyly syndromes.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW
12kDa/50kDa/79kDa
Observed MW
79kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 550-676 of human IFT81 (NP_054774.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using IFT81 antibody (144-61394) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - IFT81 antibody (144-61394)

Expiration:
12 months from the date of shipment when stored properly.