Anti-IGF1R (N-term K66) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P08069
Gene Id	3480
Gene Symbols	IGF1R
Protein Name / Synonyms	IGF1R (N-term K66)
Clonality	Polyclonal
Preparation	This IGF1R antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51-77 amino acids from the N-terminal region of human IGF1R.
Concentration (lot specific)	2.000 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

The IGF1R receptor binds insulin-like growth factor with a high affinity and plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. The protein possess tyrosine kinase activity.

Specificity

Predicted Reactivity

Mouse, Rat

Other Names

IGF1R; Insulin-like growth factor 1 receptor; Insulin-like growth factor I receptor; CD_antigen=CD221; Insulin-like growth factor 1 receptor alpha chain; Insulin-like growth factor 1 receptor beta chain

NCBI Accession #

NP_000866.1

Other Accession #

P24062; Q60751

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

154793

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; FC: 1:10-50; IF: 1:10-50

	Western blot analysis of IGF1R Antibody (N-term K66) (Cat# 102-17514) in WiDr cell line lysates (35ug/lane).IGF1R (arrow) was detected using the purified Pab.
	IGF1R Antibody (N-term K66) (Cat# 102-17514) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the IGF1R Antibody (N-term K66) for immunohistochemistry. Clinical relevance has not been evaluated.
	IGF1R Antibody (N-term K66) (Cat# 102-17514) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	Confocal immunofluorescent analysis of IGF1R Antibody (N-term K66)(Cat# 102-17514) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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