Anti-IgG2a neg. control Antibody, RPE

Catalog #: DS-MB-03587

Mouse Anti-IgG2a neg. control [+RPE]

Product Description

Specifications

Size	100 tests
Estimated Lead Time	1-2 weeks
Specificity	This product is suitable for use as a negative control reagent for the measurement of non-specific binding of mouse monoclonal antibodies of isotype IgG2a to human tissue. This antibody may not be suitable for intracellular staining on some cell types. This antibody recognizes a rat cell surface marker, and therefore cannot be used as a negative control in this species. This product is routinely tested in flow cytometry on rat splenocytes to confirm antibody activity and on human whole blood to test for suitability as a negative control. Test results have shown that This product is also suitable for use as a negative control with bovine, ovine, porcine, equine, canine, lapine and guinea-pig tissues.
Host Species	Mouse
Protein Name / Synonyms	IgG2a
Recommended Applications	Flow Cytometry
Reconstitution	Reconstitute with 1.0 ml distilled water
Shipping Type	Ambient temperature
Storage	4°C

Description

Storage/Stability

Prior to reconstitution store at +4 °C. Following reconstitution store at +4 °C.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 12 months after receipt.

Format

Supplied as:

Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized

Formulation:

Phosphate buffered saline

Reconstitution:

Reconstitute with 1.0 ml distilled water

Preparation

Immunogen:

Activated rat T-helper cells.

Preservative:

0.09%	Sodium Azide
1%	Bovine Serum Albumin
5%	Sucrose

This product

Spleen cells from BALB/c mice were fused with cells from the NS1 mouse myeloma cell line.

Expiration:

12 months from the date of shipment when stored properly.

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