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Anti-IKB alpha Antibody
Catalog #: 107-10924
IKB alpha antibody, 1 mg/ml (100 ul)
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 4792 |
| Protein Name / Synonyms | IKB alpha |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Synonyms
IKBA antibody, MAD-3 antibody, NFKBI antibody, NFKBIA antibody, NF-kappa-B inhibitor alpha antibody, IkappaBalpha antibody, I-kappa-B-alpha antibody, nuclear factor of kappa light chain gene enhancer in B-cells antibody, ikB-alpha antibody, major histocompatibility complex enhancer-binding protein MAD3 antibody, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
36 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 317 of IKB alpha (Uniprot ID# P25963)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
IKB alpha antibody detects NFKBIA protein at nucleus on U251 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U251 xenograft.
IKB alpha antibody (107-10924) dilution: 1:500.
Sample: Paraffin-embedded U251 xenograft.
IKB alpha antibody (107-10924) dilution: 1:500.
IKB alpha antibody detects IKB alpha protein by Western blot analysis.
A. 30 µg BCL-1 whole cell lysate/extract
B. 30 µg C2C12 whole cell lysate/extrac
10 % SDS-PAGE
IKB alpha antibody (107-10924) dilution: 1:5000
A. 30 µg BCL-1 whole cell lysate/extract
B. 30 µg C2C12 whole cell lysate/extrac
10 % SDS-PAGE
IKB alpha antibody (107-10924) dilution: 1:5000
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using IkappaB-alpha(107-10924) antibody at 1:200 dilution.
Sample (30 ug of whole cell lysate)
A: Hep G2
10% SDS PAGE
107-10924 diluted at 1:1000
A: Hep G2
10% SDS PAGE
107-10924 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.