3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-IKK beta Antibody
Catalog #: 107-10549
IKK beta antibody, 0.41 mg/ml (100 ul)
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Rat |
| Host Species | Rabbit |
| Gene Id | 3551 |
| Protein Name / Synonyms | IKK beta |
| Concentration (lot specific) | 0.41 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Research Area | NFkB Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Synonyms
FLJ33771 antibody, FLJ36218 antibody, FLJ38368 antibody, FLJ40509 antibody, IKK-beta antibody, IKK2 antibody, IKKB antibody, MGC131801 antibody, NFKBIKB antibody, IKBKB antibody, IKK-B antibody, I-kappa-B kinase 2 antibody, I-kappa-B-kinase beta antibody, nuclear factor NF-kappa-B inhibitor kinase beta antibody, inhibitor of nuclear factor kappa-B kinase subunit beta antibody, inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta antibody
Specificity
Species Reactivity
Human, Rat
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
87 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 493 and 739 of IKK beta (Uniprot ID# O14920)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunohistochemical analysis of paraffin-embedded Colon ca, using IKK beta(107-10549) antibody at 1:500 dilution.
IKK beta antibody detects IKK beta protein by western blot analysis.
A. 30 µg 293T whole cell extract
B. 30 µg whole cell extract of DDDDK-human IKBKB-transfected 293T cells
7.5 % SDS-PAGE
IKK beta antibody (107-10549) dilution: 1:5000
A. 30 µg 293T whole cell extract
B. 30 µg whole cell extract of DDDDK-human IKBKB-transfected 293T cells
7.5 % SDS-PAGE
IKK beta antibody (107-10549) dilution: 1:5000
IKK beta antibody detects IKBKB protein by Western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
7.5 % SDS-PAGE
IKK beta antibody (107-10549) dilution: 1:1000
A. 30 µg PC-12 whole cell lysate/extract
7.5 % SDS-PAGE
IKK beta antibody (107-10549) dilution: 1:1000
Immunofluorescence analysis of paraformaldehyde-fixed A431, using IKK beta(107-10549) antibody at 1:200 dilution.
Sample (30 ug of whole cell lysate)
A: A431
7.5% SDS PAGE
107-10549 diluted at 1:1000
A: A431
7.5% SDS PAGE
107-10549 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.