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Anti-IL-1 beta Antibody
Catalog #: RB-IP-01-101
Mouse Anti-Human IL-1 beta Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Clonality | Monoclonal |
| Immunogen | Immunogen was recombinant protein derived from human IL-1 beta. This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with the immunogen. The IgG fraction of tissue culture supernatant was purified by Protein G affinity chromatography. |
| Recommended Applications | ELISA (recommended work dilution= For Sandwich-based ELISA and membranebased assay, detecting level is up to 1pg/ml when it is used for capture), Western Blotting (recommended work dilution= 0.1-0.2 µg/ml) |
| Reconstitution | Supplied as lyophilized purified antibody. It can be reconstituted with sterile water before use. Please avoid freeze-thaw cycles. |
| Research Area | Inflammation |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
IL-1 production is generally thought to be associated with inflammation, but it is has shown to be expressed during kidney development, thymocyte differentiation and cartilage degradation. IL-1 plays a critical role in the regulation of immune response and inflammation, acting as an activator of T and B lymphocytes and natural killer (NK) cells. In T cells, IL-1 stimulates the production of IL-2 and selectively inhibits IL-4 expression. IL-1 induces B cell proliferation and maturation, and immunoglobulin synthesis. NK cells require IL-1beta for production of the anti- pathogen IFN-?. IL-1 has also been implicated in several pathological conditions including rheumatoid arthritis, inflammatory bowel disease and atherosclerosis.
Specificity
This antibody was selected for its ability to detect human IL-1beta in Sandwich ELISA and Array. In ELISA, this antibody shows approximately 5% cross-reactivity with human IL-RI, and no crossreactivity with other tested antibodies.
Storage
Store at -20°C, if not intended for use within a month, keep it at 4°C and minimize freezing and thawing when use.
References
- P.E., Webb, A.C., Rosenwasser, L.J., Mucci, S.F., Rich, A., Wolff, S.M. and Dinarello, C.A. 1985. Nucleotide sequence of human monocyte interleukin-1 precursor cDNA. Proc. Natl. Acad. Sci. USA 81: 7907-7911.
Expiration:
12 months from the date of shipment when stored properly.