RayBiotech

3607 Parkway Ln Ste 200 Norcross, Georgia 30092

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770-729-2992 | +1-888-494-8555

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Anti-IL-10 Antibody

Catalog #: 130-11162

Rabbit Anti-Human IL-10 Antibody

Product Description

Specifications

Size20 µg, 100 µg, 200 µg, 500 µg, 1 mg
Estimated Lead Time1-2 business days
SpeciesHuman
Host SpeciesRabbit
Accession Number
P22301
Gene Id
3586
Gene Symbols
IL10
Protein Name / Synonyms
Interleukin-10 (IL-10) (Cytokine synthesis inhibitory factor) (CSIF)
ClonalityPolyclonal
PreparationThis antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with the immunogen. The IgG fraction of tissue culture supernatant was purified by Protein G/A affinity chromatography.
Immunogen
Immunogen was recombinant human IL10 protein
Recommended ApplicationsELISA (recommended work dilution= 1:40, 000)
Antigen Catalog Number
230-30254
Reconstitution
Product is supplied as a powder obtained from lyophilization of purified antibody in glycine solution. Reconstitute the antibody with sterile water to a final concentration of 1 mg/ml.
Research AreaAngiogenesis, Cancer, Inflammation, Autophagy
Shipping TypeBlue ice
Storage-20°C

Description

Description

Interleukin-10 (IL-10), also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. In humans, IL-10 is encoded by the IL10 gene.

Western Blot Analysis

Ab: Rabbit Anti-IL-10 (Cat#: 130-11162) 1:1000
Ab: HRP Goat anti-rabbit IgG (H + L) 1:5000

Immunohistochemistry (IHC) Analysis

IHC showed positive staining in human colon tumor tissue. The rabbit anti-human IL-10 antibody (130-11162) was used at a 200-fold dilution, followed by mouse and rabbit IgG (ready to use) polymerization using HRP polymer, and counterstained with hematoxylin. Heat-mediated antigen retrieval was performed using Tris/EDTA buffer at pH9.0 before starting the IHC staining (40X).

Storage/Stability

Store at -20°C. If intended for use within a month, may keep at 4°C. Minimize freeze-thaw cycles.

Expiration

12 months from the date of shipment when stored properly.