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Anti-IL-10 Antibody
Catalog #: RB-IP-01-112
Mouse Anti-Human IL-10 Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | NM_000572 |
| Clonality | Monoclonal |
| Immunogen | Immunogen was recombinant protein derived from human IL-10. This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with the immunogen. The IgG fraction of tissue culture supernatant was purified by Protein G affinity chromatography. |
| Recommended Applications | ELISA (recommended work dilution= For Sandwich-based ELISA and membranebased assay, detecting level is up to 1pg/ml when it is used for capture), Western Blotting (recommended work dilution= 0.1-0.2 µg/ml) |
| Reconstitution | Supplied as lyophilized purified antibody. It can be reconstituted with sterile water before use. Please avoid freeze-thaw cycles. |
| Research Area | Inflammation |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Interleukin 10, or IL-10, is a 178 amino acid protein that is primarily secreted by TH2 clones. IL-10 has dual functions, the first of which is the suppression of cytokine production by TH1 clones responding to antigen presented by monocyte and macrophage antigen presenting cells (APCs). The second function consists of the inhibition of response of cytokine targeted cells, possibly by the downregulation of CD25 (the interleukin-2 receptor) on macrophages and B lymphocytes. Human and murine IL-10 exhibit 81% sequence identity at the amino acid level, and share 73% identity at the cDNA level. Both human and murine IL-10 are acid-liable and exist as non-covalently-linked homodimers in solution. IL-10 exerts its biological activity through the IL-10 receptor (IL-10R), a 560 amino acid, glycoprotein whose expression can be induced in cultured macro phages and fibroblasts by lipopolysaccharide (LPS) stimulation. IL-10 expression has been shown to be elevated in HIV-1 infected individuals and has been implicated in the progression of the disease.
Specificity
This antibody was selected for its ability to detect human IL-10 in Sandwich ELISA and Array. In ELISA, this antibody shows approximately 5% cross-reactivity with human IL-1b and 8, and no crossreactivity with murine IL-10 and other tested proteins.
Storage
Lyophilized: store at 4 C. After reconstitution, if not used within a month, aliquot and store at 20 C. Stability: Two years lyophilized, one month in solution at 4°C.
References
- Feng, L., Tang, W.W., Chang, J.C. and Wilson, C.B. 1993. Molecular cloning of rat cytokine synthesis inhibitory factor (IL-10) cDNA and expression in spleen and macrophages. Biochem. Biophys. Res. Commun. 192: 452-458.
Expiration:
12 months from the date of shipment when stored properly.