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Anti-IL-15 Antibody, Biotinylated
Catalog #: RB-IP-01-115B-50
Mouse anti-IL-15 Biotinylated Antibody, 50 ug
Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Mouse |
| Accession Number | NP_000576 |
| Clonality | Monoclonal |
| Immunogen | Immunogen was whole expressing mouse IL-15. This antibody was obtained from a mice immunized with the immunogen, then fusion, selection and identification. This antibody was produced from a hybridoma resulting from the fusion of a myeloma with B cells deriveded from the immunized mouse. The IgG fraction of cell culture supernatant was purified by Protein G affinity chromatography. |
| Isotype | IgG1 |
| Recommended Applications | ELISA (recommended work dilution= 1: 1, 000-4, 000), Western Blotting( recommended work dilution= 1-2 µg/ml) |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Research Area | Inflammation |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
IL-15 is produced largely by dendritic cells, monocytes, andstromal cells. The IL-15R complex consists of three chains, IL-15R , IL-2/15Rbeta, and C chains. IL-15 binds the endogenous IL-15R chain expressed by IL-15-producing cells and is trans-presented to the complex of IL-2/15Rbeta and C chains with the signaling capacity expressed on T cells. The recognition of IL-15 by the latter receptor chains leads to the activationof various signaling pathways including JAK1 and 3/STAT5 aswell as PI3K/AKT. IL-15 has diverse functions that include promoting cytotoxicity and maintenance of memory CD8+ T cells as well as proliferation and differentiationof NK and NK T cells.
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
The antibody specifically detected protein derived from human. It also recognizes tested samples containing IL-15 in both ELISA and WB. This antibody shows no cross-reactivity with mouse, rat IL-15 and other unrelated proteins.
References
- Liu K, Catalfamo M, Li Y, Henkart PA, Weng NP. IL-15 mimics T cell receptor crosslinking in the induction of cellular proliferation, gene expression, and cytotoxicity in CD8+ memory T cells. Proc Natl Acad Sci U S A. 2002.
- Kim HR, Hwang KA, Kang I. Dual roles of IL-15 in maintaining IL-7RalphalowCCR7- memory CD8+ T cells in humans via recovering the phosphatidylinositol 3-kinase/AKT pathway. J Immunol. 2007 Nov 15;179(10):6734-40.
Expiration:
12 months from the date of shipment when stored properly.