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Anti-IL-15 Antibody
Catalog #: RB-IP-01-115
Mouse Anti-Human IL-15 Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | NP_000576 |
| Clonality | Monoclonal |
| Immunogen | Immunogen was whole expressing mouse IL-15. This antibody was obtained from a mice immunized with the immunogen, then fusion, selection and identification. This antibody was produced from a hybridoma resulting from the fusion of a myeloma with B cells deriveded from the immunized mouse. The IgG fraction of cell culture supernatant was purified by Protein G affinity chromatography. |
| Recommended Applications | ELISA (recommended work dilution= 1: 1, 000-4, 000), Western Blotting( recommended work dilution= 1-2 µg/ml) |
| Reconstitution | Supplied as 100 µl of purified antibody in sterile PBS (pH 7.5), containing 0.1% BSA. The final concentration is indicated on the shipping vial. Please avoid freeze-thaw cycles. |
| Research Area | Inflammation |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
IL-15 is produced largely by dendritic cells, monocytes, andstromal cells. The IL-15R complex consists of three chains, IL-15R , IL-2/15Rbeta, and C chains. IL-15 binds the endogenous IL-15R chain expressed by IL-15-producing cells and is trans-presented to the complex of IL-2/15Rbeta and C chains with the signaling capacity expressed on T cells. The recognition of IL-15 by the latter receptor chains leads to the activationof various signaling pathways including JAK1 and 3/STAT5 aswell as PI3K/AKT. IL-15 has diverse functions that include promoting cytotoxicity and maintenance of memory CD8+ T cells as well as proliferation and differentiationof NK and NK T cells.
Specificity
The antibody specifically detected protein derived from human. It also recognizes tested samples containing IL-15 in both ELISA and WB. This antibody shows no cross-reactivity with mouse, rat IL-15 and other unrelated proteins.
Storage
Always prepare single-use aliquots planning use while receiving package. A small amount of antibody can be kept at 4°C for daily use. For a long storage, recommend to keep them at -20°C after adding an equal volume of glycerol in PBS. It will not lose activity over a year. Minimize freezing and thawing when use.
References
- Liu K, Catalfamo M, Li Y, Henkart PA, Weng NP. IL-15 mimics T cell receptor crosslinking in the induction of cellular proliferation, gene expression, and cytotoxicity in CD8+ memory T cells. Proc Natl Acad Sci U S A. 2002.
- Kim HR, Hwang KA, Kang I. Dual roles of IL-15 in maintaining IL-7RalphalowCCR7- memory CD8+ T cells in humans via recovering the phosphatidylinositol 3-kinase/AKT pathway. J Immunol. 2007 Nov 15;179(10):6734-40.
Expiration:
12 months from the date of shipment when stored properly.