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Anti-IL-6 Antibody
Catalog #: 130-10071
Mouse Anti-Human IL-6 Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | AAH15511 |
| Clonality | Monoclonal |
| Clone | 3C1-C2-D11 |
| Immunogen | Immunogen was recombinant protein derived from IL-6. This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with the immunogen. The IgG fraction of tissue culture supernatant was purified by Protein G/A affinity chromatography. |
| Recommended Applications | ELISA (recommended work dilution= 15.6 ng/ml ), Western Blotting (recommended work dilution= 1:1, 000 ), (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, protein marker, Lane 2, recombinant protein IL-6, Lane 3, 293T lysate, Lane 4, SP2/0 lysate. The membrane blot was probed with anti-IL-6 primary antibody (1 µg/ ml). Proteins were visualized using a Donkey antimouse secondary antibody conjugated to IRDye 800CW detection system. |
| Antigen Catalog Number | 230-00011 |
| Reconstitution | Product is supplied as a powder obtained from lyophilization of purified antibody in PBS without preservatives. Reconstitute the antibody with sterile water to a final concentration of 1 mg/ml. |
| Research Area | Cardiovascular Disease, Inflammation |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Interleukin-6 (IL-6) is an interleukin, secreted by T cells and macrophages to stimulate immune response to trauma. IL-6 is an anti-inflammatory cytokine mediating through its inhibitory effects on IL-1 and TNF-alpha, and its activation of IL10 and IL-1ra. Interleukin 6 interacts with IL-6 receptor and glycoprotein 130. IL-6 mediates fever and the acute phase response, and involves in many disease processes, such as prostate cancer, depression, atherosclerosis, diabetes, and rheumatoid arthritis. Metastatic cancer patients have higher blood IL-6.
Specificity
The Mouse anti-human IL-6 antibody specifically detects target derived from human recombinant protein at various concentrations. Cross reactivity with mouse and rat are expected from sequence similarity.
Storage
Store at 4°C if intended for use within one month, otherwise, store at -20°C to -80°C. The lyophilized antibody is stable for at least 18 months after the date of receipt when stored at -20°C to -80°C. After reconstitution, it can be aliquoted and stored frozen at -20°C to -80°C in a manual defrost freezer for 6 months without detectable loss of activity. Upon reconstitution, the antibody can also be stored for 1 month at 4°C. Please avoid freeze-thaw cycles, as this will lower the activity of the antibody.
References
- van der Poll T, et al. (1997). Interleukin-6 gene-deficient mice show impaired defense against pneumococcal pneumonia. J Infect Dis 176 (2): 43944.
- Kishimoto T, et al. (1995). Interleukin-6 family of cytokines and gp130. Blood 86 (4): 12431254.
- Heinrich PC, et al. (2003). Principles of interleukin-6- type cytokine signalling and its regulation. Biochem. J. 374 (Pt 1): 120.
- Taga T, et al. Interleukin-6 triggers the association of its receptor with a possible signal transducer, gp130. Cell 58 (3): 57381.
Expiration:
12 months from the date of shipment when stored properly.