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Anti-IL-6 Antibody, Biotinylated
Catalog #: 130-10060B-50
Mouse anti-IL-6 Biotinylated Antibody, 50 ug
Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Mouse |
| Accession Number | AAH15511 |
| Clonality | Monoclonal |
| Immunogen | Immunogen was recombinant protein derived from IL-6. This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with the immunogen. The IgG fraction of tissue culture supernatant was purified by Protein G/A affinity chromatography. |
| Isotype | IgG1 |
| Recommended Applications | ELISA (recommended work dilution= 15.6 µg/ml ), Western Blotting (recommended work dilution= 1:1, 000 ), (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, protein marker, Lane 2, recombinant protein IL-6, Lane 3, 293T lysate, Lane 4, SP2/0 lysate. The membrane blot was probed with anti-IL-6 primary antibody (1 µg/ ml). Proteins were visualized using a donkey antimouse secondary antibody conjugated to IRDye 800CW detection system. |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Research Area | Cardiovascular Disease, Inflammation |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Interleukin-6 (IL-6) is an interleukin, secreted by T cells and macrophages to stimulate immune response to trauma. IL-6 is an anti-inflammatory cytokine mediating through its inhibitory effects on IL-1 and TNF-alpha, and its activation of IL10 and IL-1ra. Interleukin 6 interacts with IL-6 receptor and glycoprotein 130. IL-6 mediates fever and the acute phase response, and involves in many disease processes, such as prostate cancer, depression, atherosclerosis, diabetes, and rheumatoid arthritis. Metastatic cancer patients have higher blood IL-6.
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
The mouse anti-human IL-6 antibody specifically detects targets derived from human recombinant protein at various concentrations. Cross reactivity with mouse and rat are expected from sequence similarity. The antibody specifically binds to its immunogen and did not show any cross reactivity with unrelated antigens in ELISA. The specificity for binding to recombinant protein, cellular protein and native antigen is not defined.
References
- van der Poll T, et al. (1997). Interleukin-6 gene-deficient mice show impaired defense against pneumococcal pneumonia. J Infect Dis 176 (2): 43944.
- Kishimoto T, et al. (1995). Interleukin-6 family of cytokines and gp130. Blood 86 (4): 12431254.
- Heinrich PC, et al. (2003). Principles of interleukin-6- type cytokine signalling and its regulation. Biochem. J. 374 (Pt 1): 120.
- Taga T, et al. Interleukin-6 triggers the association of its receptor with a possible signal transducer, gp130. Cell 58 (3): 57381.
Expiration:
12 months from the date of shipment when stored properly.