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Anti-IL-8/CXCL8 Antibody
Catalog #: RB-IP-01-111
Mouse Anti-Human IL-8/CXCL8 Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Specificity | This antibody was selected for its ability to detect human IL-8 in Sandwich ELISA and Array. In ELISA, this antibody shows approximately 5% cross-reactivity with human IL-1b, and no crossreactivity with murine IL-8 and other tested proteins. |
| Accession Number | NM_000584 |
| Clonality | Monoclonal |
| Immunogen | Immunogen was recombinant protein derived from human IL-8. This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with the immunogen. The IgG fraction of tissue culture supernatant was purified by Protein G/A affinity chromatography. |
| Recommended Applications | ELISA (recommended work dilution= For Sandwich-based ELISA and membrane based assay, detecting level is up to 1pg/ml when it is used for capture), Western Blotting (recommended work dilution= 0.1-0.2 µg/ml) |
| Reconstitution | Supplied as lyophilized purified antibody. It can be reconstituted with sterile water before use. Please avoid freeze-thaw cycles. |
| Research Area | Inflammation, Chemokine Biology |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Interleukin-8, or IL-8, the prototypic member of the C-X-C, or alpha, family of chemokines, is a chemoattractant cytokine involved in the chemotaxis and activation of neutrophils. IL-8 expression has been correlated to a large number of chronic inflammatory diseases, including inflammatory bowel disease (IBD) and atherosclerosis. IL-8 is cleaved from a 99 amino acid precursor to a 72 amino acid, nonglycosylated, and biologically active protein. Whether IL-8 functions in vivo as a monomer or a hydrogen-bonded dimer has not yet been resolved. Two IL-8 receptors, designated IL-8RA and IL-8RB, have been described and share 77% sequence identity. Both are seven-transmembrane domain proteins (7TMD), similar to the G protein-coupled receptors, and, in addition to IL-8, serve as receptors for other members of the alpha and beta chemokine families.
Typical Data
Storage/Stability
Lyophilized: store at 4°C. After reconstitution, if not used within a month, aliquot and store at 20°C. Stability: Two years lyophilized, one month in solution at 4°C.
Expiration
12 months from the date of shipment when stored properly.
References
- Knall, C., Young, S., Nick, J.A., Buhl, A.M., Worthen, G.S., and Johnson, G.L. 1996. Interleukin-8 regulation of the Ras/Raf/mitogen-activated protein kinase pathway in human neutrophils. J. Biol. Chem. 271: 2832-2838.